Molecular Diagnostics
General guidance about swab selection for PCR: Cotton/Dacron/Polyester Swabs are acceptable. Calcium alginate and gel swabs should not be used and, where possible, use of wooden swabs should be avoided. Swabs can be submitted dry when indicated or in appropriate medium as recommended below. Contact us for more information.
*Preferred specimen Additional fees may apply
**Additonal fees may apply, click Here for more information.
***If shipping to PSU, do not use the US Postal Service
|
Test Agent |
Site |
Price** |
Procedure |
Species |
Tissue/ Specimen |
Container |
Shipping*** |
Turn-Around |
Remarks |
|---|---|---|---|---|---|---|---|---|---|
|
Avian Influenza (AI) Including H5/H7 subtyping |
All |
$35.00 |
RRT-PCR |
Avian: chickens, turkeys, and other gallinaceous birds, wild birds, ducks |
Tracheal swab* (for gallinaceous birds), cloacal swab* (for waterfowl), intestine, lung, trachea, air sac, sinus |
Leak-proof container with VTM or BHI broth. Don't use wooden swabs |
Overnight on ice, do not freeze |
1-3 days |
Up to 5 swabs can be pooled in a tube containing BHI broth or VTM. VTM/BHI available from NBC, PVL, PSU |
|
Avian paramyxovirus-1 (APMV-1) |
All |
$35.00 |
RRT-PCR |
Avian: chickens, turkeys, and other gallinaceous birds, wild birds, ducks |
Tracheal swab* (for gallinaceous birds), cloacal swab* (for waterfowl), intestine, lung, trachea, air sac, sinus |
Leak-proof container with VTM or BHI broth. Don't use wooden swabs |
Overnight on ice, do not freeze |
1-3 days |
Up to 5 swabs can be pooled in a tube containing BHI broth or VTM. VTM/BHI available from NBC, PVL, PSU |
| Avian Reovirus PCR | All | $80.00 | |||||||
| Bluetongue (BTV) | PVL | $25.00 | |||||||
|
Bovine viral diarrhea virus (BVD) |
PSU PVL |
$35.00 each Up to 10 blood/serum pool $60.00 Ear notch pool up to 20 samples $20.00 PVL only |
RRT-PCR |
Bovine |
Serum-red top tube. Whole blood-lavender top tube. Tissues (including ear notches) |
Leak-proof container |
Overnight on ice/gel packs |
2-5 days |
Pooling of serum samples is recommended when testing whole herds. Up to 30 serum samples are pooled for cattle. Confirmation of individual positives is done at no charge by virus isolation. |
| Bovine respiratory syncitial virus (BRSV) | PSU | $35.00 | PCR | Bovine | Fresh respiratory tissues | Overnight on ice packs | |||
| Campylobacter jejuni | PVL | $35.00 | PCR | ||||||
| Chlamydophila |
PVL PSU |
$45.00 | PCR | Avian, Bovine, Caprine, Ovine | |||||
|
Clostridium perfringens toxin typing |
PSU PVL |
$35.00 |
PCR |
All species |
Feces or intestinal loop. Isolated organism |
Sterile container. Anaerobic BA in AnaPak |
Overnight on ice/gel packs |
2-8 days |
For PCR, organism needs to be cultured |
|
Eastern Equine Encephalitis (EEE) |
PVL |
$35.00 |
PCR |
All species |
Brain tissue |
Fresh or formalin fixed paraffin embedded tissue, slide mailer |
Fresh tissue, overnight shipment, on ice, do not freeze |
3-5 days |
FFPE tissue preferred with histologic lesions |
|
Encephalo-myocarditis Virus (EMCV) |
PSU |
$35.00 |
RT-PCR |
Porcine |
Heart, brain, spleen, liver, pancreas, and skeletal muscle tissue |
Fresh or formalin fixed paraffin embedded tissue |
Fresh tissue, overnight shipment, on ice, do not freeze |
3-5 days |
|
|
Equine rhino-pneumonitis herpesvirus (EHV) EHV-1 PCR EHV-4 PCR |
PVL |
|
PCR |
Equine |
15 ml blood in heparin or EDTA (lavender top tube), nasal swab, tissues including placenta-fetus, lung, nasal secretions, lymph nodes, thymus, brain. Unstained slide, FFPE tissues |
Leak-proof container. Tissue materials for virus isolation and PCR should be submitted in VTM. Slide mailer. |
Overnight shipment, on ice |
3-5 days |
For PCR only, nasal swab samples without VTM are also acceptable |
|
Escherichia coli toxin typing and virulence testing |
PSU |
$45.00 |
PCR |
All species |
Feces or other tissues |
Sterile container |
Standard delivery Overnight |
2-8 days |
|
| Herpesvirus, Koi/Carp | All | $35.00 | |||||||
| Infectious Bronchitis | All | $100.00 | Mass, Conn and Ark strains | ||||||
| Infectious Laryngotracheitis | All | $80.00 | PCR | ||||||
|
Leptospira spp |
PVL |
$20.00 |
PCR |
All species |
urine, kidney, placenta, spleen, liver |
Sterile container. Paraffin embedded tissues (FFPE) |
Overnight on ice/gel packs |
1-3 days |
Leptospirosis urine collection: For best recovery potential, inject with furosemide IV or IM. Clean vulva/prepuce and wait 10 min for second urination. Collect 10 ml urine midstream |
| Mycobacterium paratuberculosis (Johne's disease) |
PVL |
Individual $17.00 Pooled $20.00 |
PCR |
Bovine |
Feces (5-10 g) ileocecal valve, mesenteric lymph node (FFPE) blocks |
Wide-mouth screw-top container, child-resistant prescription vial, sterile container |
Overnight on ice/gel packs |
Once a week. 1-5 days |
|
|
Mycoplasma spp. |
PSU PVL |
$20.00 |
PCR AFLP-PCR |
All species |
Culture broth, plates. Tissues (1.5 in3), aspirates. Swabs |
Sterile-capped vial. Sterile container |
Overnight on ice packs |
1-5 days |
Avoid the use of calcium alginate or wooden shaft swabs |
|
Mycoplasma gallisepticum |
PSU PVL |
$20.00 |
PCR |
Avian |
Culture broth, plates. Tissues (1.5 in3), aspirates. Swabs |
Sterile-capped vial |
Overnight on ice packs |
1-5 days |
Avoid the use of calcium alginate or wooden shaft swabs |
|
Mycoplasma synoviae |
PSU PVL |
$20.00 |
PCR |
Avian |
Culture broth, plates. Tissues (1.5 in3), aspirates. Swabs |
Sterile-capped vial |
Overnight on ice packs |
1-5 days |
Avoid the use of calcium alginate or wooden shaft swabs |
|
Mycoplasma bovis |
PSU PVL |
$20.00 |
PCR |
Bovine |
Culture broth, plates. Tissues (1.5 in3), aspirates. Swabs |
Sterile-capped vial |
Overnight on ice packs |
1-5 days |
Avoid the use of calcium alginate or wooden shaft swabs |
|
Mycoplasma hyopneumoniae |
PSU |
$20.00 |
PCR |
Swine |
Culture broth, plates. Tissues (1.5 in3), aspirates. Swabs |
Sterile-capped vial |
Overnight on ice packs |
1-5 days |
Avoid the use of calcium alginate or wooden shaft swabs |
|
Neospora Caninum |
PSU PVL |
$35.00 |
PCR |
All species |
Formalin fixed paraffin embedded tissue (FFPE) or fresh tissue (heart and brain) |
Sterile-capped vial or tissue block |
Fresh tissues overnight on ice packs. Fixed tissue via standard mail |
1-5 days |
FFPE tissues preferred with histologic lesions |
|
Porcine respiratory and reproductive syndrome (PRRS) |
PSU |
$20.00 |
RRT-PCR |
Porcine |
Lung, lymphoid tissue. Serum |
Leak-proof container with or without VTM |
Overnight on ice, do not freeze |
5-6 days |
PCR is run on F |
|
Porcine Circovirus type 2 (PCV-2) |
PSU |
$35.00 |
PCR |
Porcine |
Lymph nodes, lung, spleen, tonsil. Serum |
Leak-proof container with VTM |
Overnight on ice, do not freeze |
1-5 days |
|
|
Salmonella spp. |
NBC |
$35.00 (plasmid profile $35.00) |
Real time PCR |
All species |
Feces (25 g) |
Leak-proof container |
Overnight on ice, do not freeze |
2-3 days |
|
| Salmonella genomic fingerprinting | $50.00 | PFGE | |||||||
|
Sequencing including viral, bacterial, fungal |
All |
$80.00 |
*MicroSeqTM |
All species |
Isolate(s) on plate, broth, or in suitable medium. *Sterile body fluids |
Sterile container |
Overnight on ice. *Overnight frozen |
5-15 days |
16S or MicroSeqTM for bacterial ID. 28S for fugal ID. Viral Gene sequencing |
| Streptococcus equi subspecies equi | NBC | $35.00 | PCR | ||||||
| Swine Influenza | PSU | $40.00 | Including novel H1N1 | ||||||
|
West Nile Virus (WNV) |
PVL |
$35.00 |
Real Time PCR |
All species |
Brain tissue from mammals. Kidney, brain, intestine, spleen, lung, etc., from avian spp. |
Fresh, formalin fixed tissue. Slide mailer |
For fresh tissue, overnight on ice, do not freeze |
3-5 days |
FFPE tissues preferred with histologic lesions |
Procedure codes
BHI - brain heart infusion broth
PCR - polymerase chain reaction
VTM - viral transport media
FFPE - formalin fixed paraffin embedded
RRT-PCR - real time reverse transcriptase polymerase chain reaction
