Bhushan M. Jayarao, MVSc, PhD, MPH

Program Areas

• Pre-harvest food safety (safe food production on farms)
• Zoonoses (diseases communicable between animals and humans)
• Public Health (issues related to animal production and human health)
  Specific areas
  • Antimicrobial resistance
  • Mastitis
  • Milk Quality (Bulk Tank Milk Analysis)
  • Molecular Epidemiology
  • Epidemiology of Infectious Diseases
  • Occupational Health
  • Emerging Technologies in Food Safety and Public Health (Bioreporters, Molecular Beacons, Nanobarcodes)

Memberships

• American Association of Veterinary Food Hygienists
• American Dairy Science Association
• American Veterinary Medical Association
• National Mastitis Council
• National Mastitis Research Workers Society
• Pennsylvania Veterinary Medical Association
• American Society for Microbiology

Professional Experience

• Associate Professor/Extension Veterinarian - Department of Veterinary Science, Penn State University, University Park, Pennsylvania. July 2002-Present
• Assistant Professor/Extension Veterinarian - Department of Veterinary and Biomedical Sciences, Penn State University, University Park, Pennsylvania. July 1998-June 2002
• Assistant Professor - Dairy Science Department, South Dakota State University, Brookings, South Dakota. July 1995-June 1998
• Research Assistant Professor - Department of Animal Science, The University of Tennessee, Knoxville, Tennessee. July 1991-June 1995
• Post-Doctoral Research Associate, Department of Animal Science, The University of Tennessee, Knoxville. 1989-1991
• Ph.D. Fellow, University of Veterinary Sciences, Budapest, Hungary. 1986-1989
• Assistant Professor of Veterinary Public Health, Bombay Veterinary College, Bombay, India. 1982-1986
• Veterinarian; Bandra Veterinary Clinic, Bandra, Bombay, India. 1980-1986

Awards

• American Dairy Science Association West Agro Award (2002) - Outstanding research towards the abatement of mastitis.

Publications

Jayarao, B.M.; Gillespie, B.E., Oliver, S.P. Journal of Food Protection, Volume 59, Number 6, June 1996 , pp. 615-620(6). [Article Link] - opens new window

Sawant AA, Hegde NV, Straley BA, Donaldson SC, Love BC, Knabel SJ, Jayarao BM. Antimicrobial-resistant enteric bacteria from dairy cattle. Appl Environ Microbiol. 2007 Jan;73(1):156-63. [PubMed Link] - opens new window

Straley, B. A., S. C. Donaldson, N. V. Hedge, A. A. Sawant, V. Srinivasan, S. P. Oliver, and B. M. Jayarao. 2006. Public health significance of antimicrobial-resistant gram-negative bacteria in raw bulk tank milk. Foodborne Pathog Dis. 3:222-233. [PubMed Link] - opens new window

Jayarao, B. M., S. C. Donaldson, B. A. Straley, A. A. Sawant, N. V. Hegde, and J. L. Brown. 2006. A survey of foodborne pathogens in bulk tank milk and raw milk consumption among farm families in Pennsylvania. J Dairy Sci. 89:2451-2458. [PubMed Link] - opens new window

Donaldson, S. C., B. A. Straley, N. V. Hegde, A. A. Sawant, C. DebRoy, and B. M. Jayarao. 2006. Molecular epidemiology of ceftiofur-resistant Escherichia coli isolates from dairy calves. Appl Environ Microbiol. 72:3940-3948. [PubMed Link] - opens new window

Sawant, A. A., L. M. Sordillo, and B. M. Jayarao. 2005. A survey on antibiotic usage in dairy herds in Pennsylvania. J Dairy Sci. 88:2991-2999. [PubMed Link] - opens new window

Hegde, N.V., M. L. Cook, D. R. Wolfgang, B. C. Love, C. C. Maddox, and B. M. Jayarao. 2005. Dissemination of Salmonella enterica subsp. enterica Serovar Typhimurium var. Copenhagen clonal types through a contract heifer-raising operation. J Clin Microbiol. 43: 4208-4211. [PubMed Link] - opens new window

Oliver, S. P., B. M. Jayarao, B. M. and R. A. Almeida. 2005. Foodborne pathogens in milk and the dairy farm environment: food safety and public health implications. Foodborne Pathog Dis. 2:115-129. [PubMed Link] - opens new window

Jayarao, B. M., S. R. Pillai, A. A. Sawant, D. R. Wolfgang, and N. V. Hedge. 2004. Guidelines for monitoring bulk tank milk somatic cell and bacterial counts. J. Dairy Sci. 87:3561-3573. [PubMed Link] - opens new window

Jayarao, B. M., S. R. Pillai,D. R. Wolfgang, D. R. Griswold, C. A. Rossiter, D. Tewari, C. M. Burns, and L. J. Hutchinson. 2004. Evaluation of IS900-PCR assay for detection of Mycobacterium avium subsp. paratuberculosis infection in cattle using quarter milk and bulk tank milk samples. Foodborne Pathog. Dis. 1:17-26. [PubMed Link] - opens new window

Zhang, W., B. M. Jayarao, and S. J. Knabel. 2004. Multi-virulence-locus sequence typing of Listeria monocytogenes. Appl. Environ. Microbiol. 70: 913-920. [PubMed Link] - opens new window

Gillespie, B. E., A. G. Mathew, F. A. Draughon, B. M. Jayarao, and S. P. Oliver. 2003. Detection of Salmonella enterica somatic groups C1 and E1 by PCR-enzyme-linked immunosorbent assay. J Food Prot. 66:2367-2370. [PubMed Link] - opens new window

Sharer, M. V., C. Su,N. V. Hedge, B. M. Jayarao, and L. M. Sordillo. 2003. Differential expression of the lactose transporter gene affects growth of Staphylococcus aureus in milk. J. Dairy Sci. 86:2373-2381. [PubMed Link] - opens new window

Jayarao, B. M., and D. R. Wolfgang. 2003. Bulk tank milk analysis: a useful tool for improving milk quality and monitoring udder health. Veterinary Clinics of North America. 19:75-92. [PubMed Link] - opens new window

Sawant, A. A., S. R. Pillai,and B. M. Jayarao. 2002. Evaluation of five selective media for isolation of catalase negative gram positive cocci from raw milk. J. Dairy Sci. 85: 1127-1132. [PubMed Link] - opens new window

Pillai, S. R., and B. M. Jayarao. 2002. Application of IS900 PCR for detection of Mycobacterium avium subsp. paratuberculosis directly from raw milk. J. Dairy Sci. 85:1052-1057. [PubMed Link] - opens new window

Jayarao, B. M., and D. R. Henning. 2001. Prevalence of foodborne pathogens in bulk tank milk. J. Dairy Sci. 84:2157-2162. [PubMed Link] - opens new window

Wang, L., and B. M. Jayarao. 2001. Phenotypic and genotypic characterization of Pseudomonas fluorescens isolated from bulk tank milk. J Dairy Sci.84:1421-1429. [PubMed Link] - opens new window

Pillai, S. R., E. Kunze, L. M. Sordillo, and B. M. Jayarao. 2001. Application of differential inflammatory cell count as a tool to monitor udder health. J. Dairy Sci. 84:1413-1420. [PubMed Link] - opens new window

Pillai, S. R., B. M. Jayarao, J. D. Gummo, E. C. Hue, Jr., D. Tiwari , J. R. Stabel, and R. H. Whitlock. 2001. Identification and subtyping of Mycobacterium avium subsp. paratuberculosis, and Mycobacterium avium subsp. avium by randomly amplified polymorphic DNA. Vet. Microbiol. 79:275-284. [PubMed Link] - opens new window

Jayarao, B. M., S.R. Pillai, D. R. Wolfgang, D. R. Griswold, and L. J. Hutchinson. 2001. Herd level information and bulk tank milk analysis: tools for improving milk quality and udder health. Bovine Practitioner. 35:23-37.

Su, C., I. Kanevsky,B. M. Jayarao, and L. M. Sordillo. 2000. Phylogenetic analysis of coagulase gene polymorphism of Staphylococccus aureus from bovine mastitis. Vet. Microbiol. 71:53-58.

Su C, Kanevsky I, Jayarao BM, Sordillo LM. Phylogenetic relationships of Staphylococcus aureus from bovine mastitis based on coagulase gene polymorphism. Vet Microbiol. 2000 Jan;71(1-2):53-8. [PubMed Link] - opens new window

Jayarao, B. M., and S. R. Pillai. 1999. A Meta-analytic study on the detection of Mycobacterium avium subsp. paratuberculosis from patients with Crohn’s disease. July 26, 1999. [Article Link] - opens new window

Jayarao, B. M., B.E. Gillespie, M. J. Lewis, H. H. Dowlen, and S. P. Oliver. 1999. Epidemiology of Streptococcus uberis intramammary infections in a dairy herd. J. Vet. Med [B] 46: 433-442. [PubMed Link] - opens new window

Gilliespie, B. E., B. M. Jayarao, H. H. Dowlen, and S. P. Oliver.1999. Analysis and frequencies of Bola-DRB3.2 alleles from Jersey cows by PCR-RFLP. J. Dairy Sci. 82:2049-2053. [PubMed Link] - opens new window

Jayarao, B. M., and E. K. Cassel. 1999. A study on mastitis prevention and milk hygiene practices adopted by dairy producers. Large Animal Practice.20:8-13.

Jayarao, B. M., and L. Wang. 1999. A Study on the Prevalence of Gram-Negative Bacteria in Bulk Tank Milk. J. Dairy Sci. 82:2620-2624. [Article Link] - opens new window

Gillespie B. E., B.M. Jayarao, J. W. Pankey, and S. P. Oliver. 1998. Subtyping of Streptococcus dysgalactiae and Streptococcus uberis isolated from bovine mammary secretions by DNA fingerprinting. J. Vet Med. [B]-45:585-593. [PubMed Link] - opens new window

Oliver, S. P., B. E. Gillespie, , and B. M. Jayarao. 1998. Detection of new and persistent Streptococcus uberis and Streptococcus dysgalactiae intramammary infections by polymerase chain reaction-based DNA fingerprinting. FEMS Microbiol. 160:69-73. [PubMed Link] - opens new window

Oliver SP, Jayarao BM. 1997. Coagulase-negative staphylococcal intramammary infections in cows and heifers during the nonlactating and periparturient periods. Zentralbl Veterinarmed B.44(6):355-63. [PubMed Link] - opens new window

Gillespie BE, Jayarao BM, Oliver SP. 1997. Identification of Streptococcus species by randomly amplified polymorphic deoxyribonucleic acid fingerprinting. J Dairy Sci. 80(3):471-6. [PubMed Link] - opens new window

Keane K.A.; Jayarao B.M.; Gillespie B.E.; Oliver S.P. Polymerase chain reaction-based DNA fingerprinting of Enterococcus faecium isolated from intramammary infections of dairy cows. Preventive Veterinary Medicine, Volume 23, Number 1, May 1995 , pp. 87-94(8) [Article Link] - opens new window

Matthews KR, Jayarao BM, Guidry AJ, Erbe EF, Wergin WP, Oliver SP. Encapsulation of Streptococcus uberis: influence of storage and cultural conditions. Vet Microbiol. 1994 Apr;39(3-4):361-7. [PubMed Link] - opens new window

Jayarao BM, Schilling EE, Oliver SP. Genomic deoxyribonucleic acid restriction fragment length polymorphism of Streptococcus uberis: evidence of clonal diversity. J Dairy Sci. 1993 Feb;76(2):468-74. [PubMed Link] - opens new window

Adagonda T. Sherikar, Umesh D. Karkare, Jaykumar B. Khot, Bhushan M. Jayarao and Kiran N. Bhilegaonkar. Studies on thermostable antigens, production of species-specific antiadrenal sera and comparison of immunological techniquesnext term in previous termmeat speciation. Meat Science Volume 33, Issue 1, 1993, Pages 121-136.

Matthews KR, Jayarao BM, Oliver SP. Plasmid Pattern Analysis of Staphylococcus Species Isolated from Bovine Mammary Secretions. J Dairy Sci. 1992 Dec;75(12):3318-23. [PubMed Link] - opens new window

B. M. Jayarao, J. J. E. Dore JR., and S. P. Oliver. Journal of Clinical Microbiology, Vol. 30 No. 9, Sept. 1992. p. 2235-2240. [PubMed Link] - opens new window

Matthews KR, Jayarao BM, Oliver SP. Restriction endonuclease fingerprinting of genomic DNA of Staphylococcus species of bovine origin. Epidemiol Infect. 1992 Aug;109(1):59-68. [PubMed Link] - opens new window

B. M. Jayarao, B. J. Bassam, G. Caetano-Anolles, P. M. Gresshoff, and S. P. Oliver. Journal of Clinical Microbiology, Vol. 30 No. 5, May 1992, p. 1347-1350. [PubMed Link] - opens new window

Jayarao BM, Oliver SP. Aminoglycoside-Resistant Streptococcus and Enterococcus Species Isolated from Bovine Mammary Secretions. J Dairy Sci. 1992 Apr;75(4):991-7. [PubMed Link] - opens new window

B M Jayarao, J J Doré Jr, G A Baumbach, K R Matthews and S P Oliver. Differentiation of Streptococcus uberis from Streptococcus parauberis by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism Analysis of 16S Ribosomal DNA. J Clin Microbiol. 1991 December; 29(12): 2774-2778 [PubMed Link] - opens new window

Jayarao BM, Oliver SP, Tagg JR, Matthews KR. Epidemiology and Infection, Dec. 1991; 107(3):543-55. [PubMed Link] - opens new window

Jayarao BM, Oliver SP, Matthews KR, King SH. Comparative evaluation of Vitek Gram-positive identification system and API Rapid Strep system for identification of Streptococcus species of bovine origin. Vet Microbiolo. 1991 Feb 1;26(3):301-8. [PubMed Link] - opens new window

Jayarao BM, László VG, Csák K, Milch H, Kostyák A, Kovács S, Domján H, Biró G. Acta veterinaria Hungarica, 1990;38(1-2):25-32. [PubMed Link] - opens new window

Jayarao B.M., Biró G., Kovács S., Domján H., Fábián A. Acta veterinaria Hungarica, 1989;37(1-2):39-44. [PubMed Link] - opens new window

Sherikar, A. T.; Khot, J. B.; Jayarao, B. M.; Pillai, S. R. Application of polyacrylamide-gel isoelectric focusing for identification of species of origin of raw and heat treated meats. Indian Journal of Animal Sciences 58(4): 470-486 1988. [Article Link] - opens new window