Director, Government Business Development
12212 Technology Blvd., Austin, TX 78727
Multiplexed molecular and immunoassays for detection and identification of food-borne pathogens and toxins, including Shiga toxin-producing E. coli (STEC)
The complete characterization of E. coli from food or environmental samples is a complex and multi-step process that is necessary to fully determine whether a particular sample has the capability to cause serious disease. The currently adopted approaches by the FDA and USDA involve performing a combination of different detection techniques over a number of days, including include culture isolation, immunological and molecular methods. There have been recent efforts to streamline this process by combining steps and increasing the information content of assays.
One recent effort sponsored by the FDA has resulted in the availability of a multiplexed molecular serogroup identification assay based on the Luminex xMAP® platform (xMAP STEC Serogroup ID Assay). The assay detects the presence of 11 clinically relevant STEC serogroups (“Top 6”, O91, O104, O113, O128, and O157) in an isolate. Further development is ongoing to improve this assay by adding virulence markers. This presentation will provide an overview of the current 11-plex assay and the improved assay, as well as provide some information about how the versatile and open-platform MAGPIX system might be useful in other aspects of food safety.