Serotyping of E. coli Strains
Performed using antisera generated against E. coli serogroups designated O1-O187 with the exceptions of O31, O47, O67, O72, O94, O122 as these are not designated.
Performed following the procedure published by Orskov et al. 1977. Serology, Chemistry, and Genetics of O and K antigens of Escherichia coli. Bacteriol. Rev. 41:667-710. The standard strains used are described in the paper.
Performed by PCR-RFLP analysis (Polymerase Chain Reaction- Restriction Fragment Length Polymorphism) of fliC gene responsible for flagella
Performed by the method described by Machado et al. 2000. Identification of Escherichia coli flagellar types by restriction of the amplified fliC gene. Res. Microbiol. 151:535-546
Detection of genes to determine O types and H types
- O157, O145, O121, O113, O111, O103, O45, O26 and many others