K. Sandeep Prabhu, PhD
- Post-doc, Center for Molecular Toxicology, Penn State University
- PhD, Biochemistry, VMSRF-University of Mysore, India
- MSc, Biochemistry, University of Mysore, India
- BSc, Biochemistry, Yuvaraja College, University of Mysore, India
VB SC 514 (NUTRN 514) Prostaglandins and Leukotrienes (Alternate Sp semester)
The course provides an in-depth coverage of how fatty acids are metabolized in eukaryotes by various enzymatic and non-enzymatic mechanisms. The course will emphasize on the physiological roles of these fatty acid oxidation products in general health, inflammation, and reproduction along with their cellular basis of action via receptor-dependent and -independent mechanisms. (3 Credits)
VB SC 451 Immunotoxicology of Drugs and Chemicals (Coordinator: Dr. Prabhu/Co-instructor: Dr. Na Xiong) (Fa semester)
The immunotoxicology course will focus primarily on the effects of environmental chemicals, therapeutics, and recreational drugs on the immune system. Immunomodulatory mechanisms will be examined at systemic, cellular and molecular levels. Discussions will include theory, principles, and methodology and key issues in immunotoxicology. (3 Credits)
VB SC 330 Introduction to Molecular Pharmacology (Coordinator: Dr. Adam Glick/Co-instructor: Dr. Prabhu) (Sp Semester)
Molecular interactions between drugs and their tissue receptors and possible modifications of drugs to target different receptors will be discussed. Drugs used to treat infectious disease, cardiovascular disease, immune modulators, and anti-cancer drugs will be examined for their molecular interactions. Students will understand the complexities of new drug design and development from the initial stages of laboratory development to final approval for use by the Food and Drug Administration. (3 Credits)
Research projects in our laboratory focus on the following areas:
- Effect of selenium on leukemia stem cells.
- Targeting leukemia using novel lipid mediators derived from n-3 and n-6 fatty acids.
- Redox-dependent changes in the metabolome in immune cells and its relevance in disease pathogenesis.
- Effect of selenium and selenoproteins on gut inflammation with an emphasis on eicosanoid metabolism and macrophage polarization.
- Effect of host selenoproteins in helminth infection.
- Redox regulation of erythropoiesis.
- The role of selenoproteins in HIV1 transcription.
Targeting of leukemia stem cells: In collaboration with Dr. Robert Paulson
VN Gladyshev, ES Arnér, MJ Berry, R Brigelius-Flohé, EA Bruford, RF Burk, BA Carlson, S Castellano, L Chavatte, M Conrad, PR Copeland, AM Diamond, DM Driscoll, A Ferreiro, L Flohé, FR Green, R Guigó, DE Handy, DL Hatfield, J Hesketh, PR Hoffmann, A Holmgren, RJ Hondal, MT Howard, K Huang, H-Y Kim, IY Kim, J Köhrle, A Krol, GV Kryukov, BJ Lee, BC Lee, XG Lei, Q Liu, A Lescure, AV Lobanov, J Loscalzo, M Maiorino, M Mariotti, KS Prabhu, MP Rayman, S Rozovsky, G Salinas, EE Schmidt, L Schomburg, U Schweizer, M Simonović, RA Sunde, PA Tsuji, S Tweedie, F Ursini, PD Whanger, and Y Zhang (2016) Selenoprotein gene nomenclature. J. Biol.Chem. 291:24036-2404
Lakshmikanth CL, Jacob SP, Kudva AK, Latchoumycandane C, Yashaswini PS, Sumanth MS, Goncalves-de-Albuquerque CF, Silva AR, Singh SA, Castro-Faria-Neto HC, Prabhu KS, McIntyre TM and Marathe GK (2016) Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia – like pathology in Swiss albino mice. Sci. Rep. Oct 4;6:34666. doi: 10.1038/srep34666
Prabhu KS and X. G. Lei (2016) Selenium- Supplement Information. Adv. Nutr. 7(2): 415-7.
Nelson SM, Shay AE, James JL, Carlson BA, Urban JF, and Prabhu KS (2016) Selenoprotein expression in macrophages is critical for optimal clearance of parasitic helminth Nippostrongylus brasiliensis. J. Biol. Chem. 292: (In Press).
Finch ER, Kudva AK, Quickel MD, Goodfield LL, Kennett MJ, Whelan J, Paulson RF, and Prabhu KS. (2015) Chemopreventive effects of dietary eicosapentaenoic acid supplementation in experimental myeloid leukemia. Cancer Prev. Res. (Phila). 2015 Aug 19. pii: canprevres.0050.2015. [Epub ahead of print]
Narayan V, Kudva AK, Prabhu KS. Reduction of tetrathionate by mammalian thioredoxin reductase. Biochemistry. 2015 Aug 25;54(33):5121-4. [Spotlight in: Chem. Res. Toxicol. Oct 2015]
Kudva AK, Shay AE, and Prabhu KS. (2015) Selenium and inflammatory bowel disease. Am. J. Physiol. Gastrointest. Liver Physiol. 309; G71-7
Narayan V, Kodihalli RC, Liao C, Kaushal N, Carlson BA, and Prabhu KS. (2015) Epigenetic regulation of inflammatory gene expression in macrophages by selenium. J Nutr Biochem. 26(2):138-45
Kaushal N, Kudva AK, Patterson AD, Chiaro C, Kennett MJ, Desai D, Amin S, Carlson BA, Cantorna MT, and Prabhu KS. (2014) Crucial role of macrophage selenoproteins in experimental colitis. J Immunol. 193(7):3683-92
Gandhi UH, Kaushal N, Hegde S, Finch E, Kudva AK, Kennett MJ, Jordan CT, Paulson RF, and Prabhu KS. Role of endogenous eicosanoids in the suppression of leukemia by selenium. Cancer Res. 74(14):3890-901
Vijay-Kumar M, Prabhu KS, and Rogers CJ.(2014) The Microbiome and obesity-an established risk for certain types of cancer. Cancer J. 20(3):176-80
Kudva A, Kaushal N, Mohinta S, Kennett MJ, August A, Paulson RF, and Prabhu KS. (2013) Stability, bioavailability, and hypersensitivity of omega-3 derived anti-leukemic D12-PGJ3. PLoS One. Dec 2:8(12)
Chen Y-C, Prabhu KS, and Mastro A. (2013) Dietary selenium supplementation modifies breast tumor growth and metastasis. Int J Cancer. 133; 2054-64
Flock MR, Rogers CJ, Prabhu KS, and Kris-Etherton PM. (2013) Immunometabolic role of long-chain omega-3 fatty acids in obesity-induced inflammation. Diabetes Metab Res Rev. 29; 431-45
Ravindra KC, Narayan V, Lushington GH, Peterson BR, and Prabhu KS. (2012) Targeting of Histone Acetyltransferase p300 by Cyclopentenone Prostaglandin Δ(12)-PGJ(2) through Covalent Binding to Cys(1438). Chem Res Toxicol. Feb 20; 25(2): 337-47
Hegde, S, Kaushal N, Ravindra KC, Chiaro C, Hafer KT, Gandhi UH, Thompson JT, Vanden Heuvel JP, Kennett MJ, Hankey P, Paulson RF, Prabhu KS (2011) Delta12-prostaglandin J3, an omega-3 fatty acid-derived metabolite, selectively ablates leukemia stem cells in mice. Blood. Dec. 22; 118(26):6909-19
Gandhi UH, Kaushal N, Ravindra KC, Hegde S, Nelson SM, Narayan V, Vunta H, Paulson RF, and Prabhu KS (2011) Selenoprotein-dependent Up-regulation of Hematopoietic Prostaglandin D2 Synthase in Macrophages Is Mediated through the Activation of Peroxisome Proliferator-activated Receptor (PPAR)g. J Biol Chem. 286:27471-82
Nelson SM, Lei X, and Prabhu KS (2011) Selenium Levels Affect the IL-4–Induced Expression of Alternative Activation Markers in Murine Macrophages. J Nutr. Sept.; 141:1754-61
Kaushal N, Hegde S, Paulson RF, and Prabhu KS (2011) The regulation of erythropoiesis by selenium. Antiox Redox Signal. 14:1403-12
Narayan V, Ravindra KC, Chiaro C, Carey D, Aggarwal BB, Henderson AJ, and Prabhu KS (2011) Celastrol inhibits tat-dependent human immunodeficiency virus (HIV) transcription and replication. J Mol Biol. 410(5):972-83
Chen Y-C, Sosnoski D, Gandhi UH, Novinger L, Prabhu KS*, and Mastro AM* (2009) Selenium modifies the osteoblast inflammatory stress response to bone metastatic breast cancer. Carcinogenesis. 30(11):1941-8 (*Corresponding authors)
Kalantari P, Narayan V, Henderson AJ, and Prabhu KS (2009) 15-deoxy-PGJ2 inhibits HIV-1 transactivating protein, Tat, through covalent modification. FASEB J. Aug.; 23(8):2366-73
Palempalli UD, Gandhi U, Kalantari P, Vunta H, Arner RJ, Narayan V, Ravindran A, and Prabhu KS (2009) Gambogic acid covalently modifies IkB-kinase-b subunit to mediate suppression of lipopolysaccharide-induced activation of NF-kB in macrophages. Biochem J. Apr. 15; 419(2):401-409
Kalantari P, Narayan V, Natarajan SK, Muralidhar K, Gandhi UH, Vunta H, Henderson AJ, and Prabhu KS (2008) Thioredoxin reductase-1 negatively regulates HIV-1 transactivating protein Tat-dependent transcription in human macrophages. J Biol Chem. Nov 28; 283(48):33183-90
Vunta H, Zamamiri-Davis F, Palempalli UD, Bhat D, Arner RJ, Thompson JT, Peterson DG, Reddy CC, and Prabhu KS (2007) The anti-inflammatory effects of selenium are mediated through 15-deoxy-Delta12,14-prostaglandin J2 in macrophages. J Biol Chem. June 22; 282(25):17964-73
Coleman JD, Prabhu KS, Thompson JT, Reddy PS, Peters JM, Peterson BR, Reddy CC, Vanden Heuvel JP (2007) The oxidative stress mediator 4-hydroxynonenal is an intracellular agonist of the nuclear receptor peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta). Free Radic Biol Med. Apr 15; 42(8):1155-64
Diwakar BT, E. R. Finch, C. Liao, A. E. Shay, and K. S. Prabhu (2016) The role of selenoproteins in resolution of inflammation. In Selenium: Its Molecular Biology and Role in Human Health (2016) 4th Edition. Eds. D. L. Hatfield, U. Schweizer, P. Tsuji, and V. N. Gladyshev. Springer Publications. Chapter 42, pp 499-510.
Prabhu, K.S., Kudva, A. K., and Nelson, S.M. Selenium mediates a switch in macrophage polarization in "Diversity of Selenium Function in Health and Disease" Eds. R. Brigelius-Flohe and H. Sies. CRC Press; Chapter 14, pp 293-308, 2016
Kaushal, N., Narayan, V., Gandhi, U. H., Nelson, S. M., Kotha, A.K., and Prabhu, K.S. Inflammation, oxidative stress, and antioxidants. In “Chronic Inflammation: Nutritional & Therapeutic interventions." Eds. S. Roy, D. Bagchi, and S. P. Raychaudhuri. CRC Press (New York); Chapter 19, pp 259-273, 2012.
Kaushal, N., Gandhi, U. H., Nelson, S. M., Narayan, V., and Prabhu, K. S. 2011. Selenium and Inflammation. In Selenium: Its Molecular Biology and Role in Human Health 3rd Edition. Eds. D. Hatfield, M. Berry, V. N. Gladyshev, Springer Publications. Chapter 35, pp 443-456, 2012.
National Institutes of Health (NIDDK, NCI, NCCAM), American Institute of Cancer Research, Campbell Foundation, NIH CTSI- Penn State, NIH T32 (Animals models of inflammation), and College of Agricultural Sciences, Penn State Cancer Institute, Penn State University.
Research Group Members
Svanjita Berry (Undergraduate Student)
Wanying Dai (Undergraduate Student)
Dr. Diwakar T. Bastihalli, PhD (Post-doctoral Research Scholar)
James Frisbie, Undergrad Student (Schreyer's Honors College)
Dr. Ashok H. Gopalakrishna, PhD (Post-doctoral Research Scholar)
Morgan Herold, Undergrad Student (Schreyer's Honors College)
Dr. Arvind Korwar, PhD (Post-doctoral Research Scholar)
Chang Liao, MS (Graduate Student- Pathobiology)
Alexandra Nader, Undergraduate Student (Millennium Program Scholar)
Shaneice Nettleford (Graduate Student- Pathobiology)
Dr. K. Sandeep Prabhu, PhD (Principal Investigator; Professor)
Ashley Shay, BS (Graduate Student- Molecular Medicine and NIH T32 Trainee)
Honors and Awards
Ashley Shay- NIH/NIEHS Graduate Student Travel Award
Emily Finch- AICR Travel Award
Ashley Shay- T32 Trainee Award
Dr. Avinash Kudva- Travel Award- International Symposium on Bioactive Lipids in Cancer, Inflammation, and Related Diseases, Eicosanoid Research Foundation Meeting, San Yuan, PR
Dr. Naveen Kaushal- Travel Award- International Selenium Symposium, Berlin
Emily Finch, NIH Penn State-CTSI Award
Dr. Shakira Nelson, NCI Post-doctoral Research Fellowship Award
Dr. Vivek Narayan, ASBMB Travel Award
Dr. Ujjawal Gandhi, ASBMB Travel Award
Dr. Shakira Nelson, ASBMB Travel Award
Dr. Emily Finch, PhD (Graduate Student- Immunology/Infectious Disease and CTSI Scholar); Current position: Post-doctoral fellow, St.Judes Children's Research Hospital, Memphis, TN.
Dr. Laura Goodfield, Phd (Post-doctoral Research Scholar); Current position: Scientist, Sanofi-Aventis, Boston Area
Dr. Avinash Kudva, PhD (Post-doctoral Research Scholar); Current position: Scientific Officer; Mangalore University, India
Hunter Fraker Undergrad Student (Schreyer Honors College); Current position: MD Student, George Washington University, Washington, D.C.
Dr. Vivek Narayan, PhD (Graduate Student); Current position: Post-doctoral Scholar, MGH-Harvard Medical School and Boston Children's Hospital.
Dr. Naveen Kaushal, PhD (Post-doctoral Research Scholar); Current position: Assistant Professor, Department of Biophysics, Panjab University, Chandigarh, India.
Nicole Wilski, BS (Undergrad Student); Current position: Graduate Student; Thomas Jefferson University - Microbiology & Immunology Program
Dr. Shakira Nelson (Graduate Student- Pathobiology); Current position: CPFP Post-doctoral Fellow, NCI, Bethesda
Sara Farwell (BS Student); Current position: Graduate Student, Lehigh University
Steven Cornelius (BS Honors Student); Current position: MD Student, Penn State Hershey College of Medicine
Kayleigh McCormick (BS Honors Student); Current position: DVM Student, UPENN
Dr. Ujjawal H. Gandhi, M.B.,B.S. PhD (PhD Molecular Toxicology); Current position: Chief Medical Resident, Mt Sinai Hospital, NYC
Dr. Tejo Nagaraja, PhD DBT-CREST Visting Professor; Current position: Professor, Thapar University, India.
Dr. Uma Palempalli, DBT-CREST Visiting Professor; Current position: Professor, SV University for Women, Tirupati, India.
Dr. Ravindra C. Kodihalli (Post-doctoral Scholar); Current position: Staff Scientist, WAVE Life Sciences, Cambridge, MA.
Dr. Anil Kotha, PhD (Post-doctoral Scholar); Current position: UT San Antonio, TX
Kelsey Hafer (BS Schreyer's Honors Student); Current position: Veterinarian, Women's Humane Society- Animal Hospital, Bensalem, PA
Amanda Tuchinsky (BS Schreyer's Honors Student);Current position: MD Student, Jefferson Med School
Prof. P. Reddanna (Visiting Professor); Professor and Dean- School of Life Sciences, University of Hyderabad, India
Pranav Maddali, BS (Research Tech); Current position: PhD Student; RPI- Albany, NY
Dr. Christopher Chiaro, PhD (Post-doctoral Research Scholar); Current position: Post-doc, Patterson Lab- Metabolomics Core; Penn State
Dr. Ryan J. Arner, PhD (Research Associate); Current position: DETRA-PSU Program Staff
Dr. Parisa Kalantari (Post-doc); Currently: Research Assistant Professor, Tufts University, Boston, MA
Pradeep Bodumalla (MS-Pathobiology); Current position: Unknown
Dr. Hema Vunta (PhD-Pathobiology) Currently: Lecturer, College of Pharmacy, Cincinnati, OH
Dr. K. Muralidhar (Visiting Professor) Current position: Professor of Zoology, University of Delhi, India
Stephanie Larson (MS- Pathobiology) Current position: Physician-Emergency Medicine, Erie, PA
Jessica Stahle (BS- Honors) Current position:Resident in Radiology, Department of Small Animal Clinical Science, Virgnia-Maryland College of Veterinary Medicine
Deepa Bhat (BS Honors) Current position: Senior Product Manager, Genentech
Katie Miro (BS Honors) Currently: Physician- Internal Med, Bellevue Hospital Ctr, NYC
- Immunology and Infectious Disease Research Faculty
Regulation of immune function by oxidative stress and antioxidants, including selenium. Regulation of HIV transcription by selenium.
- Molecular Toxicology & Carcinogenesis Research Faculty
Regulation of immune function by oxidative stress and antioxidants, including selenium. Regulation of HIV transcription by selenium.