Mammalian Virology

Tissues for virus isolation should be collected as aseptically as possible. Viral and chlamydial transport media are available upon request, from PADLS laboratories.

Note: Virus isolations may require several serial passages for amplification of replication and expression of viable and measurable virus in a specimen. Hence, certain virus isolations may require up to 28 days or longer. Identification of viral isolates is done using serologic or molecular techniques.

Available Virology Tests

• Bovine rotavirus – ELISA
• BVD ear notch Ag ELISA
• BVD microplate assay pi (persistent infection)
• BVD virus ELISA (serum)
• Blackleg assay (FA, 4 antibodies)
• Chlamydia isolation
• Virus isolation

Available Virology PCR Tests

• Bovine coronavirus (BCoV) PCR
• Bovine herpesvirus (BHV1) or Infectious bovine rhinotracheitis (IBR) virus PCR
• Bovine respiratory syncytial virus (BRSV) PCR
• Bovine viral diarrhea virus (BVDV) PCR (tissue/milk/serum)
• BVDV (type 1 & 2) pooled PCR serum or blood – cattle
• Encephalomyocarditis virus (EMCV) PCR
• Infectious bronchitis virus PCR
• Neospora caninum PCR
• Parainfluenza virus type 3 (PI3) PCR
• Porcine Circovirus type 2 (PCV-2) RRT-PCR
• Porcine parvovirus Gel-based PCR, RT-PCR
• Porcine respiratory coronavirus (PRCV) and transmissible gastroenteritis virus (TGEV) Duplex PCR
• Porcine respiratory and reproductive syndrome (PRRS) PCR
• Transmissible gastroenteritis virus (TGEV) -RRT-PCR

General guidance about swab selection for PCR

Cotton/Dacron/Polyester Swabs are acceptable.

Calcium alginate and gel swabs should not be used and, where possible, use of wooden swabs should be avoided.

Swabs can be submitted dry when indicated or in appropriate medium as recommended in the user guide.

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Current Fee Schedule

Test Look-up Form and User Guide